4ULV
Cytochrome c prime from Shewanella frigidimarina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.409, 58.323, 76.122 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.300 - 1.290 |
| R-factor | 0.15253 |
| Rwork | 0.151 |
| R-free | 0.18578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.322 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 1.360 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.060 | 0.740 |
| Number of reflections | 62629 | |
| <I/σ(I)> | 14 | 2.1 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.2M AM SULFATE, 0.1M HEPES PH7 |
