4UJ8

Structure of surface layer protein SbsC, domains 6-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	50.887, 54.459, 92.243
Unit cell angles	103.59, 105.23, 91.42

Refinement procedure

Resolution	23.001 - 1.700
R-factor	0.196
Rwork	0.194
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4UJ7 4uie
RMSD bond length	0.007
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.290
Number of reflections	93503
<I/σ(I)>	8.5	2.4
Completeness [%]	91.6	91
Redundancy	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1