4UJ8
Structure of surface layer protein SbsC, domains 6-7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 50.887, 54.459, 92.243 |
Unit cell angles | 103.59, 105.23, 91.42 |
Refinement procedure
Resolution | 23.001 - 1.700 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4UJ7 4uie |
RMSD bond length | 0.007 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.600 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.290 |
Number of reflections | 93503 | |
<I/σ(I)> | 8.5 | 2.4 |
Completeness [%] | 91.6 | 91 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |