4UJ7
Structure of the S-layer protein SbsC, domains 5-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.096, 43.753, 49.715 |
Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
Resolution | 48.280 - 1.540 |
R-factor | 0.19121 |
Rwork | 0.190 |
R-free | 0.21665 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iuc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.300 | 1.640 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.040 | 0.290 |
Number of reflections | 30189 | |
<I/σ(I)> | 15.7 | 3 |
Completeness [%] | 98.8 | 95.7 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |