4UIE
Crystal structure of the S-layer protein SbsC, domains 7, 8 and 9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-27 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.831, 69.831, 196.018 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.750 - 3.100 |
R-factor | 0.21993 |
Rwork | 0.216 |
R-free | 0.28970 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.205 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.180 | 0.460 |
Number of reflections | 9719 | |
<I/σ(I)> | 7.2 | 2.1 |
Completeness [%] | 91.0 | 83.6 |
Redundancy | 4.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | CA. 5MG/ML PROTEIN CONC. 2-2.4M SODIUM MALONATE PH 7.0 |