4UHO
Characterization of a Novel Transaminase from Pseudomonas sp. Strain AAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.958, 120.798, 134.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.770 - 1.240 |
| R-factor | 0.10631 |
| Rwork | 0.105 |
| R-free | 0.12314 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uhm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 1.260 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 144518 | |
| <I/σ(I)> | 12.6 | 2.8 |
| Completeness [%] | 99.9 | 98.2 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS AT 8 MG/ML, DROPS WERE 200 NL PLUS 200 NL WITH THE RESERVOIR SOLUTION MADE UP OF 10% PEG 4000, 0.2 M MGCL2, 0.1 M TRIS PH 7 |
