4UHN
Characterization of a Novel Transaminase from Pseudomonas sp. Strain AAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-28 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.912, 119.686, 134.257 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.340 - 2.210 |
| R-factor | 0.14716 |
| Rwork | 0.145 |
| R-free | 0.18843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uhm |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.280 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.270 | 1.120 |
| Number of reflections | 25807 | |
| <I/σ(I)> | 13.3 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN WAS AT 10 MG/ML AND WAS SET UP IN 200 NL PLUS 200 NL DROPS AGAINST 2.5 M NACL, 20 MM ZINC ACETATE, 0.1 M IMIDAZOLE BUFFER AT PH 8 |
