4UHK

Crystal structure of the receiver domain of CpxR from E. coli (phosphorylated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Spacegroup name	P 21 21 2
Unit cell lengths	86.831, 74.144, 76.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.380 - 2.600
R-factor	0.1885
Rwork	0.186
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.380	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.050	0.650
Number of reflections	15756
<I/σ(I)>	19.5	2.3
Completeness [%]	93.6	94.4
Redundancy	5.1	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1