4UG3
B. subtilis GpsB N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.672, 39.893, 77.277 |
| Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
| Resolution | 50.300 - 2.800 |
| R-factor | 0.2245 |
| Rwork | 0.216 |
| R-free | 0.30114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ug1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.438 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 7791 | |
| <I/σ(I)> | 10.6 | 3.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.05M BIS TRIS PH 6.5, 0.05M AMMONIUM SULPHATE, 30% PENTAERYTHRITOL ETHOXYLATE |
