4UFQ
Structure of a novel Hyaluronidase (Hyal_Sk) from Streptomyces koganeiensis.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.90753, 0.97942 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 66.884, 66.884, 483.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.244 - 1.450 |
| R-factor | 0.1508 |
| Rwork | 0.149 |
| R-free | 0.17430 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.240 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.090 | 1.000 |
| Number of reflections | 75047 | |
| <I/σ(I)> | 20.49 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19 | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTERDIFFUSION; 30% V/V PEG8K, 0.2 M AMMONIUM SULPHATE |
