4UF7
Ghanaian henipavirus (Gh-M74a) attachment glycoprotein in complex with human ephrinB2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 127.220, 152.530, 163.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.470 - 1.700 |
| R-factor | 0.17604 |
| Rwork | 0.175 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vsm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.458 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.880 |
| Number of reflections | 172833 | |
| <I/σ(I)> | 18.2 | 1.8 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 12% PEG 4000, 0.1 M SODIUM ACETATE PH 5.5, 0.1 M LISO4, AND 0.1 M NACL |
