4UF2
Deerpox virus DPV022 in complex with Bax BH3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-09 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.481, 92.481, 45.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.240 - 3.000 |
R-factor | 0.2167 |
Rwork | 0.214 |
R-free | 0.27430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DPV022_BIM |
RMSD bond length | 0.002 |
RMSD bond angle | 0.403 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.480 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.140 | 1.100 |
Number of reflections | 4297 | |
<I/σ(I)> | 10.2 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 20% PEG 6000, 0.2M MES PH 6.0, 0,2M AMMONIUM SULPHATE. |