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4UF2

Deerpox virus DPV022 in complex with Bax BH3

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron siteAustralian Synchrotron
BeamlineMX2
Temperature [K]100
Detector technologyCCD
Collection date2013-10-09
DetectorADSC QUANTUM 315r
Spacegroup nameP 41 21 2
Unit cell lengths92.481, 92.481, 45.529
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.240 - 3.000
R-factor0.2167
Rwork0.214
R-free0.27430
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)DPV022_BIM
RMSD bond length0.002
RMSD bond angle0.403
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX ((PHENIX.REFINE))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]92.4803.180
High resolution limit [Å]3.0003.000
Rmerge0.1401.100
Number of reflections4297
<I/σ(I)>10.21.7
Completeness [%]100.0100
Redundancy6.97.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1620% PEG 6000, 0.2M MES PH 6.0, 0,2M AMMONIUM SULPHATE.

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