4UF1
Deerpox virus DPV022 in complex with Bak BH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.260, 93.260, 45.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.707 - 2.300 |
| R-factor | 0.1796 |
| Rwork | 0.178 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DPV022_BIM |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.710 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 9129 | |
| <I/σ(I)> | 10.9 | 3.1 |
| Completeness [%] | 97.7 | 99 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 17% PEG 8000, 0.2M MES PH 5.5, 0.2M AMMONIUM SULPHATE |
