4UEY
Structure of the periplasmic domain PhoQ double mutant (W104C-A128C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.035, 45.371, 81.374 |
| Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
| Resolution | 31.246 - 1.900 |
| R-factor | 0.2324 |
| Rwork | 0.231 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 35731 | |
| <I/σ(I)> | 12.9 | 1.6 |
| Completeness [%] | 97.8 | 93.8 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
