4UEY
Structure of the periplasmic domain PhoQ double mutant (W104C-A128C)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.035, 45.371, 81.374 |
Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
Resolution | 31.246 - 1.900 |
R-factor | 0.2324 |
Rwork | 0.231 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.217 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.540 |
Number of reflections | 35731 | |
<I/σ(I)> | 12.9 | 1.6 |
Completeness [%] | 97.8 | 93.8 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.5 |