4UEJ
Closed state of galactitol-1-phosphate 5-dehydrogenase from E. coli in complex with glycerol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.311, 78.810, 68.244 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 41.672 - 1.740 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a2c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.190 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 10144 | |
| <I/σ(I)> | 11.2 | 3.2 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
