4UEJ

Closed state of galactitol-1-phosphate 5-dehydrogenase from E. coli in complex with glycerol.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	65.311, 78.810, 68.244
Unit cell angles	90.00, 94.75, 90.00

Refinement procedure

Resolution	41.672 - 1.740
R-factor	0.1872
Rwork	0.185
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a2c
RMSD bond length	0.007
RMSD bond angle	1.113
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.190	1.830
High resolution limit [Å]	1.740	1.740
Rmerge	0.100	0.600
Number of reflections	10144
<I/σ(I)>	11.2	3.2
Completeness [%]	99.2	99.2
Redundancy	6.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1