4UEG
Crystal structure of human glycogenin-2 catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.6, 2.3 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.113, 80.541, 73.683 |
Unit cell angles | 90.00, 118.74, 90.00 |
Refinement procedure
Resolution | 64.608 - 1.930 |
R-factor | 0.1885 |
Rwork | 0.187 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q4s |
RMSD bond length | 0.005 |
RMSD bond angle | 0.840 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.610 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.060 | 0.740 |
Number of reflections | 47488 | |
<I/σ(I)> | 14.91 | 2.31 |
Completeness [%] | 96.8 | 95.4 |
Redundancy | 5.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.3 M MGFORMATE, 0.1 M TRIS PH 8.5 WITH 30 % ETHYLENE GLYCOL IN MOTHER LIQUOR AS CRYOPROTECTANT |