4UEG
Crystal structure of human glycogenin-2 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.6, 2.3 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.113, 80.541, 73.683 |
| Unit cell angles | 90.00, 118.74, 90.00 |
Refinement procedure
| Resolution | 64.608 - 1.930 |
| R-factor | 0.1885 |
| Rwork | 0.187 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.610 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.060 | 0.740 |
| Number of reflections | 47488 | |
| <I/σ(I)> | 14.91 | 2.31 |
| Completeness [%] | 96.8 | 95.4 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.3 M MGFORMATE, 0.1 M TRIS PH 8.5 WITH 30 % ETHYLENE GLYCOL IN MOTHER LIQUOR AS CRYOPROTECTANT |
