4UEB

Complex of D. melanogaster eIF4E with a designed 4E-binding protein (Form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-02
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	38.670, 94.530, 167.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.843 - 2.520
R-factor	0.2065
Rwork	0.204
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4UE8 CHAIN A
RMSD bond length	0.004
RMSD bond angle	0.671
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.590
High resolution limit [Å]	2.520	2.520
Number of reflections	20795
<I/σ(I)>	9.4	2.1
Completeness [%]	96.2	97.1
Redundancy	4.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M MES PH 6.5, 22% PEG300