4UE9

Complex of D. melanogaster eIF4E with the 4E-binding protein 4E-T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65 2 2
Unit cell lengths	40.000, 40.000, 443.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.922 - 2.150
R-factor	0.2374
Rwork	0.235
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4UE8 CHAIN A
RMSD bond length	0.002
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.900	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	12812
<I/σ(I)>	25.6	4.5
Completeness [%]	100.0	100
Redundancy	34.7	31.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1M TRIS-HCL PH 8.5, 0.05M LISO4, 28% PEG400