4UDR
Crystal structure of the H467A mutant of 5-hydroxymethylfurfural oxidase (HMFO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-10 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.820, 122.500, 73.270 |
Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
Resolution | 73.260 - 1.600 |
R-factor | 0.17768 |
Rwork | 0.176 |
R-free | 0.21753 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.018 |
RMSD bond angle | 1.915 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.130 | 0.570 |
Number of reflections | 115432 | |
<I/σ(I)> | 4.8 | 1.5 |
Completeness [%] | 98.2 | 95.2 |
Redundancy | 2.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 22% W/V PEG3350, 200 MM MAGNESIUM FORMATE |