4UDQ
Crystal structure of 5-hydroxymethylfurfural oxidase (HMFO) in the reduced state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-19 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.550, 121.830, 72.490 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 72.470 - 1.600 |
R-factor | 0.1707 |
Rwork | 0.168 |
R-free | 0.21618 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.018 |
RMSD bond angle | 1.899 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.120 | 0.740 |
Number of reflections | 114344 | |
<I/σ(I)> | 4.8 | 1.2 |
Completeness [%] | 99.2 | 98.3 |
Redundancy | 2.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 22% W/V PEG3350, 200 MM MAGNESIUM FORMATE |