4UDE
An oligomerization domain confers pioneer properties to the LEAFY master floral regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 81.095, 81.095, 78.493 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.340 - 2.250 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.22920 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.070 | 0.510 |
| Number of reflections | 14004 | |
| <I/σ(I)> | 15.67 | 2.89 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25 MM TRIS-HCL PH 8.8, 40 MM AMMONIUM SULFATE |
