4UD5
Structural Plasticity of Cid1 Provides a Basis for its RNA Terminal Uridylyl Transferase Activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.800, 103.770, 76.390 |
Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
Resolution | 58.833 - 2.520 |
R-factor | 0.1782 |
Rwork | 0.176 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e7x |
RMSD bond length | 0.004 |
RMSD bond angle | 0.881 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1772)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.830 | 2.600 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.030 | 0.530 |
Number of reflections | 30480 | |
<I/σ(I)> | 23 | 2.1 |
Completeness [%] | 98.4 | 98.7 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20MM HEPES, 200MM NACL, 0.5MM TCEP, PH 7.0 |