4UD4
Structural Plasticity of Cid1 Provides a Basis for its RNA Terminal Uridylyl Transferase Activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.960, 62.290, 65.500 |
| Unit cell angles | 76.34, 81.08, 63.16 |
Refinement procedure
| Resolution | 45.903 - 1.740 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e7x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.065 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.030 | 0.490 |
| Number of reflections | 79749 | |
| <I/σ(I)> | 17 | 2.1 |
| Completeness [%] | 96.3 | 95.5 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20MM HEPES, 200MM NACL, 0.5MM TCEP, PH 7.0 |
