4UCX
Structure of the T18G small subunit mutant of D. fructosovorans NiFe- hydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2012-02-26 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.600, 99.270, 182.080 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.19843 |
| Rwork | 0.197 |
| R-free | 0.23068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yqw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.040 | 0.370 |
| Number of reflections | 160613 | |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | VAPOR DIFFUSION, PH 6.0, 293 K, PEG 6000, MES, IN A GLOVEBOX |
