4UCX
Structure of the T18G small subunit mutant of D. fructosovorans NiFe- hydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2012-02-26 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.600, 99.270, 182.080 |
Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.950 |
R-factor | 0.19843 |
Rwork | 0.197 |
R-free | 0.23068 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yqw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.351 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.040 | 0.370 |
Number of reflections | 160613 | |
<I/σ(I)> | 13.6 | 2.3 |
Completeness [%] | 99.2 | 99.3 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | VAPOR DIFFUSION, PH 6.0, 293 K, PEG 6000, MES, IN A GLOVEBOX |