4UCF
Crystal structure of Bifidobacterium bifidum beta-galactosidase in complex with alpha-galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.720, 101.592, 114.625 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 110.510 - 1.940 |
| R-factor | 0.15762 |
| Rwork | 0.155 |
| R-free | 0.20606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uzs |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.660 |
| Number of reflections | 153059 | |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 97.0 | 85 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 292 | 20% (W/V) POLYETHYLENE GLYCOL 3, 350, 0.2 M DIBASIC AMMONIUM TARTRATE AND 4% (W/V) 1-PROPANOL AT 292 K, pH 6 |
