4UB6
Native structure of photosystem II (dataset-1) by a femtosecond X-ray laser
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.24 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.744, 229.989, 288.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.287 - 1.950 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.23820 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.442 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 62.700 |
High resolution limit [Å] | 1.950 |
Number of reflections | 585462 |
<I/σ(I)> | 3.5 |
Completeness [%] | 98.7 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 290 | PEG, magnesium sulfate |