4UB3
DNA polymerase beta closed product complex with a templating cytosine and 8-oxodGMP, 60 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-03 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.698, 80.094, 55.358 |
Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
Resolution | 21.343 - 2.060 |
R-factor | 0.2171 |
Rwork | 0.214 |
R-free | 0.27440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.014 |
RMSD bond angle | 1.073 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.083 | 0.511 |
Number of reflections | 27186 | |
<I/σ(I)> | 16.3 | 2.1 |
Completeness [%] | 97.4 | 91.2 |
Redundancy | 4.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0 |