4UB2

DNA polymerase beta product complex with a templating cytosine and 8-oxodGMP, 120 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-09
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	55.099, 79.589, 55.668
Unit cell angles	90.00, 109.69, 90.00

Refinement procedure

Resolution	27.400 - 2.510
R-factor	0.2291
Rwork	0.226
R-free	0.29250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fms
RMSD bond length	0.005
RMSD bond angle	0.975
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.510	2.510
Rmerge	0.076	0.623
Number of reflections	25637
<I/σ(I)>	17.6	2.2
Completeness [%]	99.5	99.7
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0