4UB1
DNA polymerase beta product complex with a templating adenine and 8-oxodGMP, 90 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.131, 80.800, 55.462 |
Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
Resolution | 31.962 - 2.341 |
R-factor | 0.2278 |
Rwork | 0.222 |
R-free | 0.27720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.061 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.341 | 2.341 |
Rmerge | 0.052 | 0.334 |
Number of reflections | 34952 | |
<I/σ(I)> | 24.3 | 2.3 |
Completeness [%] | 97.3 | 77 |
Redundancy | 3.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0 |