4UAY
DNA polymerase beta product complex with a templating adenine and inserted 8-oxodGMP, 40 s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.867, 80.445, 55.391 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 31.273 - 1.984 |
| R-factor | 0.1873 |
| Rwork | 0.184 |
| R-free | 0.24690 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.956 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.060 | 0.284 |
| Number of reflections | 51562 | |
| <I/σ(I)> | 24.2 | 2.8 |
| Completeness [%] | 96.6 | 72.1 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0 |
