4UAW

DNA polymerase beta substrate complex with a templating adenine and incoming 8-oxodGTP, 0 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	50.932, 79.929, 55.515
Unit cell angles	90.00, 107.59, 90.00

Refinement procedure

Resolution	37.726 - 1.900
R-factor	0.1863
Rwork	0.183
R-free	0.24010
RMSD bond length	0.005
RMSD bond angle	0.966
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.068	0.528
Number of reflections	57082
<I/σ(I)>	28.9	4.4
Completeness [%]	98.0	97.7
Redundancy	5.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 7.5