4UAW
DNA polymerase beta substrate complex with a templating adenine and incoming 8-oxodGTP, 0 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.932, 79.929, 55.515 |
Unit cell angles | 90.00, 107.59, 90.00 |
Refinement procedure
Resolution | 37.726 - 1.900 |
R-factor | 0.1863 |
Rwork | 0.183 |
R-free | 0.24010 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.966 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.068 | 0.528 |
Number of reflections | 57082 | |
<I/σ(I)> | 28.9 | 4.4 |
Completeness [%] | 98.0 | 97.7 |
Redundancy | 5.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 7.5 |