4UAQ
Crystal structure of the accessory translocation ATPase, SecA2, from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-26 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.600, 162.090, 67.310 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 35.640 - 2.800 |
| R-factor | 0.2152 |
| Rwork | 0.211 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.640 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.436 | |
| Number of reflections | 16255 | |
| <I/σ(I)> | 14.59 | 0.604 |
| Completeness [%] | 78.3 | 29.5 |
| Redundancy | 7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | PEG 8000, tris(hydroxymethyl)aminomethane), NaCl, ethylene glycol, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) |
