4UAN
Structure of the Ssl1 laccase mutant H99Q with depleted type-2 copper ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.522, 104.274, 163.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.140 - 1.800 |
| R-factor | 0.15339 |
| Rwork | 0.151 |
| R-free | 0.19360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m3h |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.166 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.360 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.299 | |
| Number of reflections | 82406 | |
| <I/σ(I)> | 23.3 | 6.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM HEPES, pH 7.0, 1.0-1.3 M ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride |
