4UAH
Structure of the Ssl1 laccase mutant H99N with depleted type-2 copper ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97859 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.586, 104.328, 163.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.550 - 1.730 |
| R-factor | 0.17143 |
| Rwork | 0.170 |
| R-free | 0.20669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m3h |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.262 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.550 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.321 | |
| Number of reflections | 92927 | |
| <I/σ(I)> | 21.6 | 6.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Hepes pH 7, 1.0-1.3 ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride |
