4UAG
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BL19 |
| Synchrotron site | ESRF |
| Beamline | BL19 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.809, 65.809, 134.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.660 |
| Rwork | 0.190 |
| R-free | 0.22600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.800 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.041 * | 0.149 * |
| Number of reflections | 66314 | |
| <I/σ(I)> | 32.9 | 10.8 |
| Completeness [%] | 98.4 | 85.3 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 15 * | pH 7.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.8 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 100 (mM) | |
| 3 | 1 | drop | UMA | 1 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) |
