4UAE
Importin alpha 3 delta IBB in complex with Influenza PB2 Nuclear Localization Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.006 |
Spacegroup name | P 41 |
Unit cell lengths | 135.682, 135.682, 47.309 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.981 - 2.700 |
R-factor | 0.1876 |
Rwork | 0.185 |
R-free | 0.23860 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.073 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 23809 |
<I/σ(I)> | 11.1 |
Completeness [%] | 98.8 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Na cacodylate, pH 6.5 0.1 M NaCl 1.1 M ammonium sulfate |