4UAD

Importin alpha 7 delta IBB in complex with Influenza PB2 Nuclear Localization Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.972
Spacegroup name	C 1 2 1
Unit cell lengths	242.315, 38.836, 67.898
Unit cell angles	90.00, 98.03, 90.00

Refinement procedure

Resolution	14.860 - 2.420
R-factor	0.1928
Rwork	0.189
R-free	0.23100
RMSD bond length	0.003
RMSD bond angle	0.719
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.510
High resolution limit [Å]	2.420	2.420
Number of reflections	21920
<I/σ(I)>	24.9
Completeness [%]	90.0	60.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M magnesium acetate 12% PEG 8000 0.1M Na cacodylate at pH 6.5