4U9Y

Structure of the alpha-tubulin acetyltransferase alpha-TAT1/Mec-17 in complex with CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-10
Detector	RAYONIX MX225HE
Wavelength(s)	0.900
Spacegroup name	P 21 21 2
Unit cell lengths	42.849, 121.938, 37.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.426 - 2.201
R-factor	0.1967
Rwork	0.195
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vwd
RMSD bond length	0.004
RMSD bond angle	0.944
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.0)
Refinement software	PHENIX (phenix.refine: 1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.133	0.053	0.447
Total number of observations	65555
Number of reflections	10461
<I/σ(I)>	5.2
Completeness [%]	99.3	94.3	100
Redundancy	6.3	4.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES, 20% PEG 3350, 0.2M NaBr