4U9R

Structure of the N-terminal Extension from Cupriavidus metallidurans CzcP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-16
Detector	PSI PILATUS 6M
Wavelength(s)	1.03322
Spacegroup name	P 41
Unit cell lengths	58.160, 58.160, 72.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 2.170
R-factor	0.2313
Rwork	0.228
R-free	0.26610
RMSD bond length	0.012
RMSD bond angle	1.465
Data scaling software	xia2
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.010	36.010	2.230
High resolution limit [Å]	2.170	9.710	2.170
Rmerge	0.053	0.024	0.719
Rpim	0.016	0.007	0.223
Total number of observations	144365	1598	10281
Number of reflections	12757
<I/σ(I)>	26.8	80.2	4
Completeness [%]	99.8	97.4	99.7
Redundancy	11.3	10.7	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	PEG 4000, 1 mM CdSO4