4U9N

Structure of a membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-17
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	63.521, 70.681, 103.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.709 - 2.200
R-factor	0.2244
Rwork	0.224
R-free	0.24040
RMSD bond length	0.015
RMSD bond angle	0.726
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.170
Number of reflections	22128
<I/σ(I)>	14.5
Completeness [%]	95.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5	293	PEG300, HEPES, MnCl2