4U93
Crystal Structure of Hsp90-alpha N-domain Bound to the Inhibitor NVP-HSP990
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.726, 99.237, 91.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.270 - 1.550 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.270 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.081 | 0.187 |
| Number of reflections | 41892 | |
| <I/σ(I)> | 22.1 | 7.2 |
| Completeness [%] | 94.5 | 70.5 |
| Redundancy | 6.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Protein is used at 10mg/ml. Crystallant is: 10-20% (w/v) PEG2K MME, 50-200 mM magnesium chloride, 100 mM sodium cacodylate Equal ratio of protein to crystallant use for drops |
