4U8V
Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.79988 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 145.590, 161.590, 245.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.932 - 2.298 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dx5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 2.380 |
| High resolution limit [Å] | 2.298 | 2.298 |
| Rmerge | 2.398 | |
| Number of reflections | 256709 | |
| <I/σ(I)> | 11.12 | 0.84 |
| Completeness [%] | 100.0 | 99.68 |
| Redundancy | 7.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.05M ADA, pH 6.5, 0.2 M (NH4)2SO4, 8% PEG4000, 10% Glycerol |
