4U8T

Crystal structure of YTH domain of Zygosaccharomyces rouxii MRB1 protein in complex with N6-Methyladenosine RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9790
Spacegroup name	P 61 2 2
Unit cell lengths	117.390, 117.390, 380.928
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.921 - 2.700
R-factor	0.2009
Rwork	0.199
R-free	0.24520
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.336
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.9_1675))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.137	0.105	0.498
Total number of observations	186118
Number of reflections	41339
<I/σ(I)>	6.6
Completeness [%]	94.2	89.8	96.1
Redundancy	4.5	4.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		277	0.8 M ammonium dihydrogen phosphate