4U88
Structure of the DNA-Binding Domain of the Response Regulator SaeR from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2014-05-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.966, 61.024, 116.759 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.521 - 2.355 |
R-factor | 0.201 |
Rwork | 0.194 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ixa |
RMSD bond length | 0.009 |
RMSD bond angle | 1.163 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.350 |
Number of reflections | 9992 |
<I/σ(I)> | 19.7 |
Completeness [%] | 99.6 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 5% v/v Tacsimate, pH 7.0, 0.1M HEPES pH 7.0, 10% w/v Polyethylene glycol monomethyl ether 5000 |