4U7Y

Structure of the complex of VPS4B MIT and IST1 MIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 6 2 2
Unit cell lengths	78.401, 78.401, 67.642
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.820 - 2.502
R-factor	0.2276
Rwork	0.225
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wr0
RMSD bond length	0.007
RMSD bond angle	0.997
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.820
High resolution limit [Å]	2.500
Rmerge	0.112	0.387
Number of reflections	4455
<I/σ(I)>	21.1	6.4
Completeness [%]	96.9	98.2
Redundancy	12.3	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	299	2.58 M Na-malonate