4U7U
Crystal structure of RNA-guided immune Cascade complex from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 111.412, 118.143, 225.873 |
| Unit cell angles | 92.23, 93.55, 106.06 |
Refinement procedure
| Resolution | 43.797 - 3.003 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL |
| Data scaling software | HKL-2000 |
| Phasing software | RESOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.105 | 0.039 | 0.625 |
| Total number of observations | 680117 | ||
| Number of reflections | 216153 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 98.8 | 99.4 | 98.3 |
| Redundancy | 3.1 | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 11%-13% PEG4000, 100mM Tris-HCl, 200mM NaAc, 100mM Glycine, 50mM LiAc |
