4U7S
Homodimeric Single Domain Antibody (sdAb) against Staphylococcal enterotoxin B (SEB) Crystallized for 5 months
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2012-04-25 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.776, 72.978, 117.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.660 - 2.070 |
R-factor | 0.23267 |
Rwork | 0.232 |
R-free | 0.24207 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tyu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.079 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.660 | 2.160 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.066 | 0.196 |
Number of reflections | 17180 | |
<I/σ(I)> | 24.28 | 5.88 |
Completeness [%] | 94.9 | 79.1 |
Redundancy | 7.79 | 2.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 290 | 0.1 M Citric acid, pH 4.1, 20% Glycerol, 1.6M Ammonium sulfate |