4U7N
Inactive structure of histidine kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 31 |
Unit cell lengths | 91.599, 91.599, 97.901 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.387 - 3.200 |
R-factor | 0.2546 |
Rwork | 0.251 |
R-free | 0.28480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u7o |
RMSD bond length | 0.008 |
RMSD bond angle | 1.170 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.084 | |
Number of reflections | 36426 | |
<I/σ(I)> | 37.594 | 2 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 5.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | Ammonium sulfate, PEG 4000 |