4U7N
Inactive structure of histidine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 31 |
| Unit cell lengths | 91.599, 91.599, 97.901 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.387 - 3.200 |
| R-factor | 0.2546 |
| Rwork | 0.251 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u7o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.170 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.084 | |
| Number of reflections | 36426 | |
| <I/σ(I)> | 37.594 | 2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | Ammonium sulfate, PEG 4000 |
