4U7I
Structure of the complex of Spartin MIT and IST1 MIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97828 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 46.257, 46.257, 115.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.610 - 1.794 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dl1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.942 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | |
| High resolution limit [Å] | 1.790 | |
| Rmerge | 0.081 | 0.584 |
| Number of reflections | 12400 | |
| <I/σ(I)> | 43 | 4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 8.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 2.9 M Na-malonate |
