4U7I

Structure of the complex of Spartin MIT and IST1 MIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97828
Spacegroup name	P 43 21 2
Unit cell lengths	46.257, 46.257, 115.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.610 - 1.794
R-factor	0.1916
Rwork	0.190
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dl1
RMSD bond length	0.006
RMSD bond angle	0.942
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610
High resolution limit [Å]	1.790
Rmerge	0.081	0.584
Number of reflections	12400
<I/σ(I)>	43	4
Completeness [%]	99.6	100
Redundancy	8.1	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	2.9 M Na-malonate