4U75
HsMetAP (F309M) in complex with Methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.586, 77.398, 48.658 |
| Unit cell angles | 90.00, 90.59, 90.00 |
Refinement procedure
| Resolution | 17.700 - 1.940 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.21800 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.849 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.000 |
| High resolution limit [Å] | 1.930 |
| Number of reflections | 25066 |
| <I/σ(I)> | 2.64 |
| Completeness [%] | 95.5 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15% PEGMME, 100mM HEPES PH 6 |
