4U71
HsMetAP(F309M) IN COMPLEX WITH 1- amino(cyclohexyl)methy)phosphonic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-28 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.355, 77.248, 48.434 |
Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.19500 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.026 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.520 |
High resolution limit [Å] | 1.800 |
Number of reflections | 32202 |
<I/σ(I)> | 3.18 |
Completeness [%] | 99.4 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15% PEGMME, 100mM HEPES PH 6 |