4U71

HsMetAP(F309M) IN COMPLEX WITH 1- amino(cyclohexyl)methy)phosphonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	47.355, 77.248, 48.434
Unit cell angles	90.00, 90.92, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.159
Rwork	0.157
R-free	0.19500
RMSD bond length	0.023
RMSD bond angle	2.026
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.520
High resolution limit [Å]	1.800
Number of reflections	32202
<I/σ(I)>	3.18
Completeness [%]	99.4
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	15% PEGMME, 100mM HEPES PH 6